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. 2004 Apr;24(8):3227–3237. doi: 10.1128/MCB.24.8.3227-3237.2004

FIG. 2.

FIG. 2.

(A) Structures of TEL deletion mutants. The HLH and the ETS domains are shown by shaded and hatched boxes, respectively. Numerals are amino acid numbers in the TEL protein. (B) ERK-dependent size shifts of wild-type TEL and its deletion mutants. COS-7 cells were transfected with 5 μg of pME18S-FLAG-TEL (lanes 1 and 2), pME18S-FLAG-ΔHLH-TEL (lanes 3 and 4), pME18S-FLAG-ΔHLH+5′ID-TEL (lanes 5 and 6), pME18S-FLAG-ΔHLH+ID-TEL (lanes 7 and 8), pME18S-FLAG-Δ5′ID-TEL (lanes 9 and 10), or pME18S-FLAG-ΔETS-TEL (lanes 11 and 12) alone (lanes 1, 3, 5, 7, 9, and 11) or in combination with 5 μg of ERK expression plasmid (lanes 2, 4, 6, 8, 10, and 12), serum starved, and either left untreated (lanes 1, 3, 5, 7, 9, and 11) or treated with recombinant human EGF for 5 min (lanes 2, 4, 6, 8, 10, and 12). Western analysis was performed with anti-FLAG antibody. Positions of size markers (in kilodaltons) are shown.