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. Author manuscript; available in PMC: 2014 Dec 10.
Published in final edited form as: J Control Release. 2013 May 9;172(2):10.1016/j.jconrel.2013.04.023. doi: 10.1016/j.jconrel.2013.04.023

Figure 1.

Figure 1

A) Structure of DAPI (4’,6-diamidino-2-phenylindole dihydrochloride), propidium iodide (PI, 3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide), and acridine orange (AO, 3,6-bis(dimethylamino)acridinium chloride). B) Size exclusion chromatography of CPMV-DAPI, CPMV-PI, and CPMV-AO shows the typical elution profile of intact CPMV, co-elution of the dyes indicates loading. C) UV/visible spectra of CPMV-DAPI, CPMV-PI, and CPMV-AO showing the CPMV typical peak at 260 nm and the dye-specific absorbance peak at 358, 493, and 470 nm, respectively. D) Native agarose gel electrophoresis of CPMV and eCPMV after incubation with DAPI, PI, AO. The gels were visualized and documented under UV light and then stained with Coomassie blue and photographed under white light.