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. Author manuscript; available in PMC: 2014 Dec 10.
Published in final edited form as: J Control Release. 2013 May 9;172(2):10.1016/j.jconrel.2013.04.023. doi: 10.1016/j.jconrel.2013.04.023

Figure 3.

Figure 3

The fate of CPMV-DAPI in HeLa cells. Time and temperature-dependency: CPMV-DAPI was incubated with HeLa cells at 4°C versus 37°C for 10 min versus 60 min. A, C, E, G = CPMV channel (in green), B, D, F, H = overlay of CPMV channel (in green), cell membrane stain (WGA, in red), and DAPI in blue. Concentration dependence: I-L showing cells after incubation with different concentrations of free DAPI versus CPMV-DAPI, cell membrane in red, DAPI in blue (no CPMV staining). Colocalization: M = overlay of DAPI in blue (note in this experiment cells were stained using free DAPI, and not DAPI delivered through CPMV), endolysosomes (Lamp-1 marker) in red, and CPMV in green, N = Lamp-1 only in red, O = CPMV only in green, P = colocalization analysis using ImageJ and colocalization highlighter plug-in, colocalized signals are shown in white. The scale bars are 50 μm in length.