FIG. 1.

Expression of K15 keratin in human epidermal basal keratinocytes. Northern blot analysis of RNA from cultured primary human keratinocytes (1), total epidermis (2), separated epidermal differentiated keratinocytes (3), or basal keratinocytes (4) are shown. Five-microgram aliquots of RNA were resolved by electrophoresis through a formaldehyde agarose gel. After brief staining with ethidium bromide to visualize the rRNA bands, the gel was processed for Northern blot analysis. The blot was successively hybridized with [³²P]dCTP-labeled DNA probes derived from cDNAs encoding K15, K14, and hypoxanthine phosphoribosyltransferase (internal control).