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. 2004 Apr;24(8):3150–3156. doi: 10.1128/MCB.24.8.3150-3156.2004

FIG. 2.

FIG. 2.

Expression and functional characterization of hSVCT2 and its short isoform. (A) Western blot analysis of the expression of full-length hSVCT2 and its short isoform. 293T cells were transfected with 4 μg of a plasmid carrying full-length hSVCT2 or its short isoform. The membrane was probed with commercially available antibodies against hSVCT2. Molecular masses are in kilodaltons. (B) Uptake studies in 293T cells. Cells were untransfected or transfected with full-length hSVCT2 (SVC2) or its short isoform (SVCT2-s). Uptake of radiolableled AA was performed at different time intervals. (Inset) Accumulation of ascorbic acid during longer incubations. (C) Sodium dependency of AA uptake mediated by hSVCT2. Cells were transfected with full-length hSVCT2, and uptakes were done with (+) and without (−) sodium in the buffer at 0 and 10 min. The ratios between accumulated AA at 10 min and that at 0 min are shown. The error bars indicate standard deviations.