Table 2.
Serum ferritin, glutathione concentration, redox ratio (GSH/GSSG), and GSH-related enzyme activities (GST, GR, GPx) of normal subjects and β-thalassemia/Hb E patients (mean ± SEM).
| Subjects | Serum | Glutathione and GSH-related enzymes | |||||||
|---|---|---|---|---|---|---|---|---|---|
| ferritin | Hb | Total GSH | GSH | GSSG | GSH/GSSG | GST | GR | GPx | |
| (pmol/L) | (g/L) | (µmol/L) | (µmol/L) | (µmol/L) | (U/mg Hb) | (U/mg Hb) | (U/g Hb) | ||
| Normal subjects (n = 27) | 174 ± 14 | 143.6 ± 3.0 | 896.0 ± 46.2 | 804.4 ± 42.0 | 91.6 ± 6.4 | 9.5 ± 0.6 | 5.6 ± 0.5 | 4.6 ± 0.2 | 31.5 ± 1.5 |
| β-thalassemia/Hb E (n = 25) | 3255 ± 482 | 71.6 ± 2.7 | 607.1 ± 39.3 | 285.2 ± 26.3 | 321.9 ± 18.6 | 0.9 ± 0.1 | 12.5 ± 0.8 | 7.7 ± 0.6 | 60.7 ± 2.2 |
| P value | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
|
| |||||||||
| Splenectomized (n = 8) | 5156 ± 1112 | 70.6 ± 6.3 | 487.2 ± 54.0 | 198.2 ± 48.6 | 288.9 ± 26.3 | 0.7 ± 0.1 | 10.4 ± 1.6 | 8.9 ± 1.2 | 58.8 ± 4.6 |
| Nonsplenectomized (n = 17) | 2361 ± 319 | 72.0 ± 2.8 | 663.5 ± 46.8 | 326.1 ± 26.7 | 337.4 ± 23.9 | 1.0 ± 0.1 | 13.5 ± 0.9 | 7.1 ± 0.7 | 61.6 ± 2.5 |
| P value | 0.004 | 0.817 | 0.033 | 0.020 | 0.231 | 0.024 | 0.079 | 0.186 | 0.577 |
GPx: glutathione peroxidase; GR: glutathione reductase; GSH: glutathione; GSSG: glutathione disulfide; GST: glutathione-S-transferase.