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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Immunol. 2013 Sep 20;191(9):10.4049/jimmunol.1301625. doi: 10.4049/jimmunol.1301625

Figure 1. NK cells in aged mice fail to expand and up-regulate KLRG1 in response to pathogen-derived products.

Figure 1

Young (4-6 mo.) and aged (20-22 mo.) were individually identified by ear punches and challenged with intra-peritoneal injection of 10 μg of LPS (A and B) or 100 μg of poly I:C (C and D). Twenty micro-liters of blood samples were analyzed before, 3 and 6 days after the challenge by flow cytometry. (A and C) NK cells were identified as NK1.1+ and CD3/CD5/CD19 triple-negative cells in gated CD45+ cells. Gated NK cells are shown to demonstrate the proportion of KLRG1+ cells. (B and D) The mean and standard deviation of the number of NK and KLRG1+ NK cells per microliter of blood are presented. Data are representative of 2 experiments (n = 4). * indicates statistical significance between young and aged mice with P <0.05.