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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Immunol. 2013 Sep 20;191(9):10.4049/jimmunol.1301625. doi: 10.4049/jimmunol.1301625

Figure 7. Bone marrow stem cells from young mice fail to reverse the aging-related defect in NK cell maturation.

Figure 7

Lineage-depleted BM cells from young (2 months) CD45.1+CD45.2+ mice were transferred into anti-CD117-treated aged (20 months) CD45.1-CD45.2+ recipients. (A) Blood samples from the recipients were analyzed 1 week, 3 weeks and 3 months after bone marrow transfer. (B) Splenocytes from the recipients were analyzed 3 months after bone marrow transfer. NK cells are identified as in Figure 1. Donor-derived and host NK cells are identified by their expression of CD45.1 and CD45.2 in gated NK cells. Gated donor-derived and host NK cells are shown to demonstrate the proportion of CD27+ and CD27- cells. (C) Percent correction of aging-related CD27+ NK cell population shift by donor young HSCs was calculated (see text) for each host. The mean and standard deviation of the percent correction by donor young HSCs are presented. Data are representative of 3 experiments (n = 2)