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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Dev Biol. 2013 Aug 3;382(1):10.1016/j.ydbio.2013.07.026. doi: 10.1016/j.ydbio.2013.07.026

Figure 1. Morpholino targeting of the zebrafish chd7 RNA induces an aberrant transcript.

Figure 1

(A) Schematic of the human CHD7 and zebrafish Chd7 proteins with the location of the predicted protein domains. (B) Schematic of the zebrafish un-spliced chd7 transcript (upper) and the exon/intron splice site targeted by the chd7 morpholino (black bar). Schematic of the chd7 morphant transcript (lower) with the location of the predicted induced nonsense mutation (black arrow). Chromatogram of the sequenced morphant transcript PCR product reveals a nonsense mutation (red highlight). (C) Agarose gel with the amplified PCR products of both wild-type and morphant chd7 transcripts from Std control morphants and chd7 morphants. (D) Graph of qRT-PCR data quantifying the expression of the chd7 morphant transcript. Error bars represent standard error of the mean (SEM) (n = 4). Significance was determined by a Student’s two-tailed t-test and significant p-values are noted p < 0.01 (**).