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. 2004 Apr;24(8):3213–3226. doi: 10.1128/MCB.24.8.3213-3226.2004

FIG. 3.

FIG. 3.

Effects of deletions of homologous recombination genes on the viability of rrm3 sgs1 and rrm3 srs2 mutants. (A, B, and C) Tetrads from diploids heterozygous for rrm3, sgs1, and either rad55, rad52, or rad54 were dissected and analyzed for the presence of auxotrophic markers (rrm3::URA3, sgs1::HIS3, and rad::TRP1). (D, E, and F) Tetrads from diploids heterozygous for rrm3, srs2, and either rad55, rad52, or rad54 were dissected and analyzed for the presence of auxotrophic markers (rrm3::URA3, srs2::HIS3, and rad::TRP1). Circles indicate rrm3 sgs1 and rrm3 srs2 mutants; diamonds indicate rrm3 sgs1 rad and rrm3 srs2 rad mutants; squares indicate rad52, rad54, and rad55 single mutants; pentagons indicate rrm3 rad54 mutants; hexagons indicate srs2 rad54 mutants. (F) Since srs2 rad54 spores and rrm3 srs2 rad54 spores are inviable, it was not possible to assign the genotypes with certainty when both spore types were present in the same tetrad; only one possible assignment is shown here. (G) Doubling times of the mutant strains and appropriate controls in rich medium (YPD) are shown with standard deviations. Deletion of RAD51 or RAD55 fully suppressed the growth defects of sgs1 rrm3 and srs2 rrm3 mutants, whereas deletion of RAD52 partially rescued these mutants.