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. 2013 Oct 28;2(10):e77. doi: 10.1038/oncsis.2013.40

Figure 6.

Figure 6

TRPC3/TRPC6 silencing results in loss of macroH2A1 function. (a) Cell proliferation assays were carried out in quadruplicate using cells depleted of TRPC3, TRPC6 and/or macroH2A1 as indicated. Each bar represents the mean s.d. of four replicates in three independent experiments. (b) Cell invasion assays were performed using cells depleted of TRPC3, TRPC6 and/or macroH2A1. Each bar represents the mean s.d. of three replicates in two independent experiments. *P<0.05; **P<0.01; ***P<0.001. (c) Model of Trpc3/Trpc6 gene regulation by macroH2A and HDAC1/HDAC2. In response to repressive cellular signals, macroH2A is incorporated into the Trpc3 and Trpc6 loci. The non-histone domain of macroH2A physically interacts with HDAC1 and HDAC2, and this interaction is proposed to recruit HDAC1 and HDAC2 to the Trpc3 and Trpc6 loci. This leads to histone deacetylation and Trpc3/Trpc6 gene silencing. See DISCUSSION for more details.