FIG. 5.
Acetylated arm 6 peptide competes for β-catenin/Tcf binding more efficiently than its nonacetylated counterpart. Acetylated or nonacetylated arm 6 peptide at increasing concentrations was incubated with in vitro-translated Tcf4. GST-arm 1-12 and 32P-labeled Tcf probe were added to the reaction for EMSA analysis, followed by autoradiography. The relative intensities of the β-catenin/Tcf4/DNA complex in the corresponding lanes were quantified by the Syngene Photo Image System (Syngene, Cambridge, United Kingdom) and are shown in bar graphs in the bottom panel.