Figure 6.

Effect of different metals on glutaredoxin mutants growth. (A) Sensitivity of glutaredoxin mutants to metals. Tolerance of WT, SGRXA, SGRXB, SGRXC, SGRXAB, SGRXAC, SGRXBC, and SGRXABC strains to cadmium, copper, and nickel was examined. Tenfold serial dilutions of a 1 μg chlorophyll mL⁻¹ of exponentially growing cells suspension were spotted onto BG11C supplemented with 2 μM Cd, 3 μM Cu, and 5 μM Ni. Plates were photographed after 5 days of growth. (B) Sensitivity of glutaredoxin mutants to selenium. Tolerance of WT, SGRXA, SGRXB, SGRXC, SGRXAB, SGRXAC, SGRXBC, and SGRXABC strains to selenate (Na₂SeO₄) or selenite (Na₂SeO₃) was examined. Ten-fold serial dilutions of a 1 μg chlorophyll mL⁻¹ of exponentially growing cells suspension were spotted onto BG11C supplemented with 30 μM Na₂SeO₃ or 30 μM Na₂SeO₄. Plates were photographed after 5 days of growth.