. 2013 Aug 21;34(18):2695–2704. doi: 10.1002/elps.201300151

Table 2.

Comparison of nitrated recombinant Bet v 1a samples derived from reaction mixtures containing different ratios between PN and Tyr, respectively

Peak Nr^a)	Allergen variant^b)	Theor. mass M_r	1:1 Nitration sample^c)			5:1 Nitration sample^d)

			t_m[s]	Exp. mass/error	Relative	t_m[s]	Exp. mass/error	Relative
				M_r [ppm]	intensity^e)		M_r [ppm]	intensity
1	Bet v 1a	17439.600	970.7	17439.933/19.1	100	1007.3	17439.891/16.7	100
2	1× nitro #1	17484.598	995.4	17484.910/17.8	17	1031.1	17484.821/12.8	18
3	1× nitro #2	17484.598	1007.3	17484.878/16.0	4	1046.6	17485014/23.8	4
4	1× nitro #3	17484.598	1022.0	17484.937/19.4	11	1061.3	17484.923/18.6	17
5	2× nitro #1	17529.600	1033.9	17529.927/18.9	2	1073.2	17529.769/9.6	4
6	2× nitro #2	17529.600	1047.6	17529.939/19.6	5	1086.0	17529.987/22.1	20
7	3× nitro #1	17574.598	1054.9	17575.111/29.4	2	1093.3	17574.953/20.2	8
8	2× nitro #3	17529.600	1064.1	17529.959/20.7	1	1107.1	17529.889/16.5	3
9	3× nitro #2	17574.598	1089.7	17575.275/38.7	2	1132.7	17574.994/22.5	11
10	4× nitro #1	17619.596	1097.0	17620.928/75.8	1	1140.0	17620.076/27.2	9
11	5× nitro #1	17664.594	n.d.^f)	n.d.	n.d.	1148.2	17664.886/16.5	4
12	5× nitro #2	17664.594	n.d.	n.d.	n.d.	1180.3	17665.300/40.0	1
13	6× nitro #1	17709.592	n.d.	n.d.	n.d.	1205.0	17710.600/56.9	1

^a)

Peak numbers refer to annotations given in Fig. 5.

^b)

Variants #1–3 refer to nBet v1a variants of nominally identical mass, but different t_m (see Fig. 5).

^c)

and d) Refer to nitrated Bet v 1a samples derived from reaction mixtures containing PN:Tyr ratios of 1:1 and 5:1, respectively.

^e)

Refers to Bet v 1a of the respective sample that is assigned 100%.

^f)

Not determined since the S/N in BPE was too low to allow for unambiguous identification.