Overexpression of miR-26a promotes differentiation and proliferation of postnatal pancreas in vivo. (A) Expression of miR-26a in adult pancreas from miR-26a TG (TG) and WT mice. (B) Number of islets in P10 pancreas of WT and TG mice, as determined by counting the total number of islets from serial sections of whole pancreas. (C) Hematoxylin and eosin (H&E) images of P10 pancreas from miR-26a TG or WT mice. Islets are outlined in dots. (Scale bars, 100 µm.) (D) Expression of miR-26a in islet and nonislet fractions from P4 versus P7 WT mice. (E) Comparison of TETs and TDG gene expression in P4 and P7 islets from WT and TG mice. (F) Quantification of 5hmC, as determined by dot blot analysis, in P4 and P7 islets from WT and TG mice. (G–I) Expression of endocrine (G), progenitors or mature beta cells (H), and cell proliferation (I) markers in P7 islets of WT and TG mice. Data are shown as mean ± SD; *P < 0.05, **P < 0.01, and ***P < 0.001.