Fig. 5.
Overexpression of miR-26a enhances differentiation of pancreatic progenitor-like cells in vitro. (A) Schematic of ring colony formation. CD133+ cells were sorted from dissociated pancreata of miR-26a TG or WT mice, and plated in Matrigel-containing colony assay without exogenous R-Spondin1 (RSPO1). Ring colonies formed, consisting of mostly duct-like cells. (B) Heat map of microfluidic qRT-PCR analysis of gene expression in individual ring colonies that were handpicked 3 wk postplating. Each column indicates a signal colony. Amy 2A, amylase 2A; Cpa1, carboxypeptidase A1; Gcg, glucagon; Ins1, insulin 1; Ins2, insulin 2; Ngn3, neurogenin 3; Pdx1, pancreas and duodenal homeobox 1; Ppy, pancreatic polypeptide; Sst, somatostatin. (C) Relative gene expression (ΔCt) compared with β-actin as shown in Fig. 5B. Each column indicates a single colony.