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. 2013 Oct 16;110(44):17957–17962. doi: 10.1073/pnas.1221535110

Fig. 1.

Fig. 1.

ILT4 inhibitory receptor is expressed on peripheral blood neutrophils and neutrophil-like PLB-985 cells. (A) Flow cytometry analysis of CD11b, CD15, and CD66b markers, and ILT2, ILT4, and KIR2DL4 inhibitory receptors (open histograms) expression on neutrophils. Gray-filled histograms represent isotype controls. Data are representative of at least five experiments. (B) ILT4 expression on neutrophils by confocal microscopy. Neutrophils were stained for ILT4 (green) and nucleus (DAPI, blue). (Scale bars: 20 μm.) Images are representative of three independent experiments. (C) CD11b and ILT4 expression on undifferentiated, DMF-, and DMSO-differentiated PLB-985 cells. Percentages of positive PLB-985 cells for CD11b or ILT4 or CD11b and ILT4 are presented in each quadrant. Histograms on the right represent percentages of CD11b+/ILT4+ PLB-985 cells (n = 4). (D) CD11b or ILT4 surface expression during 5 d of differentiation of PLB-985 cells with DMSO. Curves are representative of three independent experiments. ***P < 0.001.