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. 2013 Oct 16;110(44):17957–17962. doi: 10.1073/pnas.1221535110

Fig. 6.

Fig. 6.

The up-regulation of ILT4 expression on neutrophils mediated through exocytosis is inhibited in sepsis conditions. (A) (Left) Neutrophil apoptosis was measured by Annexin V-FITC/PI staining after incubation with 5% FCS, healthy plasma (HP), or septic plasma (SP). Percentages are indicated in each panel. (Right) Quantifications of apoptotic (Annexin V-FITC+) neutrophils (n = 8). (B) ILT4 (n = 13), CD11b (n = 8), or CD66b (n = 6) expression (MFI) on neutrophils after 15 min incubation in 5% healthy plasma (white bars) or 5% septic plasma (black bars). (C) The fMLF induction of ILT4 (n = 13), CD11b (n = 8), and CD66b (n = 6) surface expression on neutrophils incubated in 5% healthy plasma (white bars) or 5% septic plasma (black bars). (D) Comparison of fMLF-induced ILT4 exocytosis on neutrophils from healthy (n = 27) and septic patients (n = 4). *P < 0.05; **P < 0.01; ***P < 0.001.