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. 2013 Oct 14;110(44):17790–17795. doi: 10.1073/pnas.1316412110

Fig. 1.

Fig. 1.

Identification of the drought-hypersensitive dwa1 mutant in rice, in which functional complementation of dwa1 restored the drought resistance. (A) Schematic diagram of DWA1 gene structure with the T-DNA insertion and genotyping of the mutant. h, heterozygote; H, homozygote; LB, left border; F and R, forward and reverse genomic primers; MS, moderate sensitivity; R, resistant to drought; RB, right border; S, sensitive to drought; VP, vector primer. (B) RT-qPCR expression analysis of DWA1 in WT and mutant tissues. L, leaf tissue; P, panicle. (C) Increased drought sensitivity of the dwa1 mutant at the seedling stage. (D) Survival rate comparisons of WT and mutant plants after drought stress. (E) Water loss rate of WT and mutant plants. (F) DWA1-FC functional complementation plants restored drought resistance. (G) Survival rate of DWA1-FC and dwa1 plants after drought stress treatment. (H) Recovery of DWA1-FC plants from reduced plant height of dwa1 at the reproductive stage.