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(A) Structure of DUPA modified with short ethylene glycol (Tet, 1), long ethylene glycol (Und, 2), Phthal (3), and DNP (4) linkers with a terminal aminooxy group. (B) Structure of p-acetylphenylalnine (pAcF) and oxime ligation of pAcF containing αCD3 Fab with P–linkers. (C) FACS of the P-αCD3 conjugates (100 nM) on Jurkat (CD3+) and C4-2 (PSMA+) cell lines. Different P–linkers are conjugated to the same residue (HC-138) of the αCD3 Fab.
