TABLE 1.
Primers and probes used in the study
| Name | Acc no.a | Globin locus coord.b | Forward primer | Reverse primer | Probe |
|---|---|---|---|---|---|
| bG59.8 | U01317.1 | 59861-59834 | TGGAAAAGCAACCCCTGC | AACTATGGATCCTTCTCTTGTGTTGG | GCTGCAGATACCATCATCCTGGCTTCAA |
| bG61.3 | U01317.1 | 61274-61301 | ACAGAGGCTTTTTGTTCCCCC | GGTAATCAGTGGTGTCAAATAGGAGG | GACACTCTTGCAGATTAGTCCAGGCAGA |
| bG63.5 | U01317.1 | 63581-63608 | GGACAGCAAGAAAGCGAGCT | TCAGAAAGTGGTGGCTGGTG | GCTAATGCCCTGGCCCACAAGTATCACT |
| bG63.7 | U01317.1 | 63702-63740 | TTTATCTTATTTCTAATACTTTCCCTAATCTCTTT | CCTTAACCCAGAAATTATCACTGTTATTC | TCAGGGCAATAATGATACAATGTATCATGCCTCT |
| bG64.1 | U01317.1 | 64157-64131 | AGTGGCCAGGCGAGGAG | ACCACCTTTCCCCTGAAGTGT | AACCATCTCGCCGTAAAACATGGAAGG |
| bG64.3 | U01317.1 | 64257-64278 | GGCATGGTTTGACTGTCCTGT | TGCAGATTCCGGGTCACTG | CCCTTCTTCCCTGCCTCCCCCA |
| bG65.3 | U01317.1 | 65356-65332 | TGAGTAATAGTTTCCTGATTCTCCCA | AAAGTCACTCTCATGGAAACAGACA | CCCCAACCCCTGGAAACCATACCTC |
| Hygromycin resistance | AGCACGAGATTCTTCGCCC | TGATCGAAAAGTTCGACAGCG | CCGAGAGCTGCATCAGGTCGGAG | ||
| LacZ | GATGTGCGGCGAGTTGC | ACCGACATCGCAGGCTTCT | AGTTTCTTTATGGCAGGGTGAAACGCAGG | ||
| CKNLYZC | AF410481 | AGATCAACAGCCGCTGGTG | CACGGGATGTTGCACAGGT | ATGGCAGGACCCCAGGCTCCAG | |
| CKNLYZNG | AF410481 | TCCAGCAGGCCAAAGAGTCT | GGATACCATCTTACCTTGTTGAGAAAT | CTGAATGTTGTGTTGCCGGAGACCTG |
a
GenBank sequence accession number.
b
Coordinates of globin locus.