Fig. 4.

GR isoform-specific patterns of domain requirements. Effect of point mutations in functional domains on transcriptional regulation differs for GRα and GRγ. U2OS cells stably expressing GR variant as indicated were treated with 100 nM dex for 4 h and relative expression levels for treated and untreated cells were analyzed by RT-qPCR for (A) SPINK5L3, and for (B) PNLIP. Averages ± SEM are shown (n = 3).