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Heparin, polyphosphate, and fucoidan block the inhibition of FVaAR prothrombinase by TFPIα. Prothrombinase activity assays were performed as in Fig. 5C with heparin (0.5 units/mL) (A), polyphosphate (150 µM) (B), or fucoidan (10 nM) (C) included in the incubation mixture (dashed lines). In all panels, data from Fig. 5C (solid lines) are shown for reference.
