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Colonies obtained following transfection of Oct4–ΔPE-GFP ES cells with an anti-FGF4 antibody population, after 3 d of growth in differentiation medium ES-Cult/N227. (A–C) typical colonies obtained in the absence of doxycycline under the indicated conditions. (D) Example of colony retaining an undifferentiated phenotype, obtained in the presence of doxycycline (1 μg/mL) (magnification 20×).
