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. 2013 Sep 30;110(44):17802–17807. doi: 10.1073/pnas.1312062110

Fig. 4.

Fig. 4.

Selected anti-FGF4 clones maintain Oct4-ΔPE-GFP expression in liquid differentiation in the presence of doxycycline. (A) Cells from the αFGF4_A and αFGF4_C clones were analyzed by flow cytometry after 7-d growth in differentiation conditions in the absence or presence of doxycycline (1 μg/mL) or in the presence of 2 μM control Erk kinase inhibitor PD0325901 (PD03). (B) Phase and fluorescent images of Oct4–ΔPE-GFP cells expressing the α-FGF4A antibody after 7 d of differentiation in N227 in the presence or absence of doxycycline. The differentiation experiment was performed two times with similar results (magnification 20×).