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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Pain. 2013 Jul 20;154(11):10.1016/j.pain.2013.07.024. doi: 10.1016/j.pain.2013.07.024

Figure 1. The effects of GABA receptor agonists and antagonists on paw withdrawal responses in mice.

Figure 1

A & B: In normal (naïve) mice, a single i.t. injection of bicuculline (GABAA antagonist, 0.5 or 1 μg [A]) or CGP43681 (GABAB antagonist, 0.25 or 0.5 μg [B]) dose-dependently increased paw withdrawals to von Frey (vF #3.0) stimuli, which lasted up to 1.5 h. Vehicle injection did not affect response rates. C & D: SNL resulted in significantly increased response rates to vF # 3.0 stimuli from pre-surgical levels (P). Four days after surgery, i.t. injection of either muscimol (GABAA agonist, 0.05 or 0.1 μg [C]) or baclofen (GABAB agonist, 0.03 or 0.06 μg [D]) dose-dependently decreased paw withdrawal responses up to 1.5 h. Vehicle treatment did not affect response rates. Data are presented as means ± SEM. P, pre-surgical time; L5 Lig, time of L5 spinal nerve ligation; i.t., intrathecal injection; *, the value significantly (p < 0.05) different from that of the vehicle control by two-way ANOVA with one repeated factor (time) followed by the Holm-Sidak post-hoc test.