Skip to main content
. Author manuscript; available in PMC: 2013 Nov 4.
Published in final edited form as: Anticancer Res. 2010 Mar;30(3):785–792.

Figure 2.

Figure 2

Treatment with CDDO-Me induces apoptosis in colorectal cancer cells. A: Binding of annexin V-FITC. HT-29 and HCT-15 cells were treated with CDDO-Me at concentrations of 1.25 to 5 μM for 20 h. Cells were then reacted with 5 μl of annexin V-FITC reagent for 30 min at room temperature. The percentage of annexin V-FITC positive tumor cells was determined by flow cytometry. Results are presented as percentage of annexin V-FITC-binding cells. B: Cleavage of PARP-1. Cells were treated with CDDO-Me as described above and PARP-1 was analyzed by immunoblotting. Each experiment was repeated at least twice.