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. 2013 Oct;54(5):489–495. doi: 10.3325/cmj.2013.54.489

Figure 1.

Figure 1

Proliferating cell nuclear antigen (PCNA) expression in single cells of the limb bud. (A) PCNA signal (arrow) in the forelimb bud cultivated in Eagle’s minimum essential medium (MEM) with rat serum. Internal negative control (arrowhead). DAB, contrasted with hematoxylin (HE). Histology of limb buds. Differentiation in 13 days old rat limb buds before cultivation (B, C, D E, F, G). (B) Forelimb bud, HE. (C) Hindlimb bud, HE. (D) Immature superficial epithelium in a hindlimb bud, HE. (E) Myotubes in a forelimb bud (arrows), HE. (F) Single hemangioblasts (arrow) and their aggregates (arrowhead) in a hindlimb bud, HE. (G) Capillary in a hindlimb bud (arrow), HE. Differentiation of rat hindlimb buds cultivated in MEM with serum for two weeks (H, I). (H) Limb bud covered by keratinized stratified epithelium. A myotube in the mesenchyme (arrow), Masson. (I) Limb bud with differentiated cartilage (asterisk), Masson.