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. 2013 Oct;54(5):419–429. doi: 10.3325/cmj.2013.54.419

Figure 4.

Figure 4

Quantitative analysis of double immunofluorescence staining in the cortex of Tg2576 and wild type (WT) mice. (A) The percentage of glial fibrillary acid protein (GFAP), Ox-6, or NeuN-positive cells in plaque, interplaque regions, and WT cortex expressed as percentage of all cortical cells. The number of GFAP-positive and Ox-6-positive cells around amyloid plaques was significantly higher than in interplaque and WT cortical tissue. On the contrary, the number of NeuN-positive cells decreased around plaques (two-way ANOVA followed by Tukey multiple comparison test, +significantly higher than in interplaque tissue and WT cortex, P < 0.05; *significantly lower than in interplaque tissue and WT cortex; P < 0.001, (A). (B) The percentage of GFAP, Ox-6, or NeuN-positive cells stained with Syt IV antibody in plaque, interplaque regions, and WT cortex. The vast majority of GFAP- and Ox-6-positive cells surrounding amyloid plaques were not positive for up-regulated Syt IV. There were no astrocytes or microglial cells positive for Syt IV in interplaque tissue or in WT cortex. All NeuN-positive cells were Syt IV-positive in all cortices. (B). Bars represent the standard deviation of the mean; Tg2576 mice (n = 4) and WT mice (n = 4).