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. Author manuscript; available in PMC: 2014 Oct 14.
Published in final edited form as: Cancer Cell. 2013 Sep 19;24(4):10.1016/j.ccr.2013.08.019. doi: 10.1016/j.ccr.2013.08.019

Figure 5. IKK inhibition decreases proliferation, increases apoptosis and induces differentiation of MLL leukemia cells.

Figure 5

(A) GSEA plot shows downregulation of cell cycle process related genes in mouse MLL-AF10 leukemia cells treated for 24 hr with IKK inhibitor versus vehicle treated cells.

(B) Mouse MLL-AF10 leukemia cells were cultured in the presence of 2 μM IKK inhibitor IV or 0.5 μM IKK inhibitor VII for 2 days, and BrdU incorporation was quantified by flow cytometry analysis.

(C) GSEA plot shows upregulation of apoptosis related genes in mouse MLL-AF10leukemia cells treated for 24 hr with IKK inhibitor versus vehicle treated cells.

(D) Mouse MLL-AF10 leukemia cells were cultured in the presence of 1 μM IKK inhibitor IV or 0.5 μM IKK inhibitor VII for 3 days. The annexin-V positive and PI negative populations constitute early apoptotic cells.

(E) GSEA plots show upregulation of monocyte or granulocyte fingerprint genes in mouse MLL-AF10 leukemia cells treated for 24 hr with IKK inhibitor versus vehicle treated cells.

(F) Light microscopy of May-Grunwald/Giemsa-stained mouse MLL-AF10 leukemia cells after 2 days of IKK inhibitor IV treatment (2 μM).

(G) Quantification of leukemia cell populations with indicated morphological features after 2 days of IKK inhibitor IV treatment (2 μM).

(H) Flow cytometry analysis of Mac-1 and Gr-1 surface expression by mouse MLL-AF10 leukemia cells after 2 days of 2 μM IKK inhibitor IV treatment.

See also Figure S5 and Table S3.

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