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. Author manuscript; available in PMC: 2014 Oct 14.
Published in final edited form as: Cancer Cell. 2013 Sep 19;24(4):10.1016/j.ccr.2013.08.019. doi: 10.1016/j.ccr.2013.08.019

Figure 8. Promoter occupancies of MLL wild-type and oncoprotein are dependent on IKK/NF- κB signaling.

Figure 8

(A) ChIP was performed on mouse MLL-AF10 leukemia cells with antibodies against Rela, HA (MLL-AF10), and control IgG. Genomic regions amplified by qPCR are indicated relative to the transcription start site (TSS) on Meis1 and Hoxa9 genes. Ey-globin and H4 promoter primers were used for negative controls.

(B) Promoter sequence of Meis1 and Hoxa9. NF-κB consensus sequence is indicated in capital letters.

(C) Mouse MLL-AF10 leukemia cells were cultured in the absence or presence of IKK inhibitors (IV or VII) for 2 days. ChIP was performed using antibodies against Rela, HA (MLL-AF10), and control IgG.

(D-F) Mouse MLL-AF10 leukemia cells were cultured in the absence or presence of IKK inhibitor VII for 2 days. ChIP was performed using antibodies against H3K79me2 and control IgG (D), Mll-c term (E), and H3K4me3 (F) and qPCR primers amplifying the indicated genomic regions. All error bars represent SD of triplicate analyses.

See also Figure S8.

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