Figure 5.

MTS proliferation assay following prolonged cilengitide treatment in combination with ionizing radiation. Cells were treated in the same manner as in Figure 3, with the addition that for the radiation groups, cells were treated with the indicated dose of IR following 1 hr of cilengitide treatment. All four cell lines show a combination effect where cilengitide in combination with radiation has an enhanced growth delay effect when compared to IR alone treated cells. A) T-47D cells show this combination effect at lower doses of cilengitide than other cells lines. B) MCF-7 cells show an improvement over radiation alone with cilengitide combination. C) MDA-MB-231 cells show a trend toward an enhanced effect of cilengitide in combination with IR treatment. D) MDA-MB-468 cells also show only a trend toward increased growth delay in the combination groups versus the radiation alone group. Figures show Mean ± SEM and represent the average of three experiments. * = ≤ 0.05.