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. 2013 Oct 22;12:30. doi: 10.1186/1476-0711-12-30

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Copyright © 2013 Zheng et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Duplex real-time PCR 10-fold dilution series. E: Efficiency; R²: coefficient of determination. Standard curves (Ct plotted against the log of the starting quantity of template for each dilution) and amplification curves of the dilution series for (A, B)bla_NDM and (C, D)bla_KPC.