Figure 6. Colonization with P. copri dominates the colonic microbiome and exacerbates local inflammatory responses.

(A) DNA was extracted from fecal pellets of media-gavaged mice and P. copri-gavaged mice 2 weeks after colonization and assayed by QPCR with P. copri specific primers compared to universal 16S. (B) Relative abundance of bacterial families in fecal DNA from media-gavaged and P. copri-colonized mice (shown in duplicate) by high-throughput 16S sequencing (regions V1–V2, 454 platform). (C) C57BL/6 mice colonized with P. copri (n = 15) or media alone (n = 13) controls were exposed to DSS for seven days and percent of starting body weight is shown. Composite data from three representative experiments are shown. (D) Representative colonoscopic images of mice colonized with P. copri or media gavage following DSS-induced colitis. Endoscopic colitis score for five individual animals is displayed. (E and F) Gross pathology (E) and histology (F) of colons from mice colonized with P. copri or media gavage following DSS-induced colitis.

DOI: http://dx.doi.org/10.7554/eLife.01202.023

Figure 6—figure supplement 1. P. copri colonization exacerbates chemically induced colitis.

(A) DNA was extracted from fecal pellets of media, P. copri, and B. thetaiotamicron gavaged mice 2 weeks after colonization and assayed by QPCR with P. copri or Bacteroides specific primers compared to universal 16S amplicon. (B) C57BL/6 mice colonized with P. copri (n = 10) or B. theta (n = 10) were exposed to DSS for seven days and percent of starting body weight is shown. (C) Percent of total CD4⁺ T-cells in the colonic lamina propria expressing IL-17 (Th17) or IFNγ (Th1) following PMA/ionomycin stimulation or expressing Foxp3 (Treg).

DOI: http://dx.doi.org/10.7554/eLife.01202.025