. 2013 Oct 2;23(12):1531–1546. doi: 10.1093/glycob/cwt085

Table I.

Monosaccharide composition of 2-AB labeled N-glycans from rhFVIIa, deduced from ESI-MS and LC-ESI-MS data analysis

Chromatographic data		Detected masses				Monosaccharide composition					Glycan name
RT (min)	Relative amount (%)	Experimental m/z	Theoretical m/z	Adduct	Deviation (ppm)	Hex	HexNAc	dHex	NeuAc	P-value	Glycan name
30.9	0.7	1234.4844	1234.4832	[M + 1H]⁺¹	−1.0	3	3	0	0	0	A₁G₀
43.7	1.5	1355.51	1355.5094	[M + 1H]⁺¹	−0.4	5	2	0	0	0	Man₅
49.8	0.3	1558.5894	1558.5888	[M + 1H]⁺¹	−0.4	5	3	0	0	0	Man₄A₁G₁
54.9	1.4	844.3196	844.3193	[M + 2H]⁺²	−0.4	4	3	0	1	0	A₁G₁S₁
57.7	1.1	881.338	881.3377	[M + 2H]⁺²	−0.3	5	4	0	0	0	A₂G₂
61.3	0.6	945.8601	945.859	[M + 2H]⁺²	−1.2	4	4	0	1	0	A₂G₁S₁
65.3	1.6	925.3457	925.3457	[M + 2H]⁺²	0.0	5	3	0	1	0	Man₄A₁G₁S₁
66.1–67.8	10.9	900.8075	900.8076	[M + 2H]⁺²	0.1	6	3	0	0	1	Man₆-P-GlcNAc
72.3	43.1	1026.8854	1026.8854	[M + 2H]⁺²	0.0	5	4	0	1	0	A₂G₂S₁
74.1–77.0	2.9	1006.3725	1006.3721	[M + 2H]⁺²	−0.4	6	3	0	1	0	Man₅A₁G₁S₁
78.3	7.0	1099.9142	1099.9144	[M + 2H]⁺²	0.2	5	4	1	1	0	A₂G₂S₁F_p
78.9	3.6	981.8341	981.834	[M + 2H]⁺²	−0.1	7	3	0	0	1	Man₇-P-GlcNAc
83.5	3.6	1099.9148	1099.9144	[M + 2H]⁺²	−0.4	5	4	1	1	0	A₂G₂S₁F_d
87.0	9.4	1172.4335	1172.4331	[M + 2H]⁺²	−0.3	5	4	0	2	0	A₂G₂S₂
89.2	2.0	1172.9404	1172.9433	[M + 2H]⁺²	2.5	5	4	2	1	0	A₂G₂S₁F_pF_d
91.3	3.0	1123.3574	1123.3568	[M + 2H]⁺²	−0.5	7	4	0	0	2	Man₇-(P-GlcNAc)₂
92.6	2.6	1245.4624	1245.4621	[M + 2H]⁺²	−0.2	5	4	1	2	0	A₂G₂S₂F_p
98.9	4.7	1228.9224	1228.9214	[M + 2H]⁺²	−0.8	7	4	0	1	1	Man₆-P-GlcNAc-A₁G₁S₁

Relative abundances (%) were deduced from integration of fluorescence signals recorded after HILIC chromatography.