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. 2012 Sep 4;5(9):925–943. doi: 10.3390/ph5090925

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

GSK3 activity in VSMC treated with butyrate and TSA. Proliferating VSMC were treated with butyrate or TSA for indicated periods of time. Cell lysates were prepared and subjected to western analysis for phosphorylated GSK3α/β (Ser21/Ser9), together with total GSK3α/β using appropriate antibodies (A) The results were quantitated and ratio of phosphorylated to non-phosphorylated total GSK3 was determined; (B) Data are presented as mean ± S.D. from three independent experiments. Analysis of the data indicates no significant difference in GSK3 activity between untreated and treated groups.