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Cyclin D1 gene expression in butyrate and TSA treated VSMC. VSMC were treated with 5 mM butyrate or 0.5 µM TSA for 48 h and total RNA was isolated and subjected to real-time RT-PCR to quantitate cyclin D1 gene transcription. 18S ribosomal RNA was used as a normalizing control. Data are presented as mean ± S.D. from three independent experiments. ** p < 0.001 compared to control VSMC.
