FIG. 3.

(a) Western blot analysis of muscle protein lysates prepared from TIS7^+/+, TIS7^+/−, and TIS7^−/− mice with muscle-specific markers. (b) Northern blot analysis of total mRNA prepared from TIS7^+/+, TIS7^+/−, and TIS7^−/− mice with muscle-specific markers. GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (c) Immunofluorescence analysis of soleus muscle for laminin expression in TIS7^+/+ and TIS7^−/− mice. Fixed cryosections were stained with respective laminin antibodies. Note the reduced expression of laminin-α2 but the lack of effect on laminin-α1 in the soleus muscle from TIS7^−/− mice. Bar, 50 μm.