TABLE 1.
Analysis of putative C/EBPβ target genesa
| Gene | Description | KO/WTb |
|---|---|---|
| MCSFR | CSF receptor | 0.96 |
| GMCSFR-α | CSF 2 receptor α (low affinity) | 0.62 |
| IL-1Rγ | IL-1 receptor, γ chain | 0.93 |
| TGF-β | TGF β-1 | 0.75 |
| IGF-I | Insulin-like growth factor I | 0.17 |
| TNFSF13B | B-cell activating factor | 3.11 |
| BAK | BCL2 homologous antagonist | 0.56 |
| Bax-a | BCL2-associated X protein | 0.76 |
| Bag-1 | BCL2-associated athanogene 1 | 1.00 |
| A1 | B cell leukemia/lymphoma 2-related protein | 3.86 |
| MCL-1 | Myeloid cell leukemia sequence 1 | 1.36 |
| Cyt C | Cytochrome c | 1.07 |
| RBP1 | RBP I isoform III | 1.14 |
| RP105 | Lymphocyte antigen 78 | 1.36 |
| PAK | P21-activated kinase 1 | 2.14 |
| MDM-2 | Mouse 3T3 cell double minute 2 | 0.60 |
| TRP53 | Transformation-related p53 | 0.86 |
| CBP | CREB binding protein | 1.40 |
| DP1 | Transcription factor DP1 | 0.37 |
| CDC2 | Cell division cycle 2 homologue A | 1.10 |
| ODC | Ornithine decarboxylase | 1.11 |
| Apex/Ref-1 | Apurinic/apyrimidinic endonuclease | 1.01 |
| Ctsd | Cathepsin D | 1.20 |
a
Total RNA from J2-WT1 and J2-KO1 cell lines was used to prepare radiolabeled cDNA probes that were hybridized to a mouse apoptosis-survival gene array (R&D Systems). The hybridization signals were quantitated by phosphorimaging and normalized to GAPDH.
b
Normalized values from J2-KO1 cells were divided by J2-WT1 values to calculate relative expression in the two cell lines (KO/WT). This experiment was performed twice with similar results. Note that IGF-I expression decreased nearly sixfold in J2-KO1 cells.