Skip to main content
. 2013 Feb 6;6(2):223–250. doi: 10.3390/ph6020223

Figure 1.

Figure 1

Intratracheal route of siRNA administration into the lungs in vivo studies. (a) Intratracheal route: under anesthesia, the trachea is exposed surgically, and a tube or needle is inserted through an incision made between the tracheal rings. Complications, such as vascular injury and air leakage, are possible due to the tracheotomy. (b) Endotracheal route: siRNAs are sprayed directly from the mouth into the lungs using a MicroSprayer® aerolizer (Penn-Century, Philadelphia, PA, USA) and a laryngoscope. It is important to maintain a clear view of the trachea during the procedure.