FIG. 7.
Cell cycle defects in swm1Δ mutant cells. Elutriated G1 cells from CLB2-myc12 (Z693) and swm1Δ CLB2-myc12 (Z2591) strains grown at 25°C were released at 37°C. (A) The percentages of budded cells (squares), of cells with a short spindle in an undivided nucleus (open circles), of cells with an elongated spindle and a divided nucleus (filled circles), and of cells with staining from Clb2-myc12 (triangles) were determined in samples taken every 15 min. (B) Cellular DNA content and mean cell volume. (C) Clb2-myc12 (green) and tubulin (red) were detected by indirect immunofluorescence, and DNA was stained with 4′,6-diamidino-2-phenylindole (DAPI, blue) in samples taken 120 min (wild-type) and 135 min (swm1Δ) after release.