FIG. 9.
Swm1/Apc13 is required for timely Pds1 degradation and sister chromatid separation. Elutriated G1 cells from cdc15-2 (wild-type, Z2804) (A), swm1Δ cdc15-2 (Z2803) (B), and swm1Δ mad2Δ cdc15-2 (Z2922) (C) strains grown at 22°C were released at 37°C. All strains contained PDS1-myc18 and a ura3 locus marked with GFP. Pds1-myc18 was detected by indirect immunofluorescence. Top panels, the percentages of budded cells (squares), of cells with staining from Pds1-myc18 (filled triangles), and of cells with separated sister chromatids (filled diamonds) were plotted over the mean cell volumes. Arrows mark separated sisters in 50% of cells. Bottom panels, cellular DNA content and the mean cell volume measured at different times after release.