FIG. 6.

Normal expression of APRIL/PAL31, an LANP family member, in LANP null mice. Western blotting of mouse cerebellar extracts from three pairs of wild-type and LANP null littermates reveals the absence of LANP staining in LANP null mice (A). Persistence of APRIL/PAL31 is observed with two different antibodies generated against rat (APRIL r) and human (APRIL h) versions of this protein (B and C, respectively). Note that APRIL h recognizes mouse APRIL and LANP, causing a doublet pattern in wild-type lanes (C). (D) A Western blot against GAPDH serves as a loading control. (E) Immunohistochemical localization of APRIL with antibody APRIL r from a LANP null cerebellum shows significant staining in the nuclei of Purkinje cells.