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. 2004 Apr;24(8):3277–3285. doi: 10.1128/MCB.24.8.3277-3285.2004

FIG. 7.

FIG. 7.

Effect of rad24Δ mutation on association of Rad9 with DSBs. (A) Phosphorylation of Rad9 in rad24Δ mutants after HO expression. Wild-type (WT) (KSC1520) and rad24Δ mutant (KSC1532) cells expressing Rad9-HA were transformed with pGAL-HO. Cells were then subjected to immunoblotting analysis as described for Fig. 2A. Tubulin was detected as a control. (B) Association of Rad9 with the HO-induced DSB in rad24Δ mutants. Wild-type (KSC1520) and rad24Δ mutant (KSC1532) cells expressing Rad9-HA were transformed with pGAL-HO and subjected to a chromatin immunoprecipitation (IP) assay as described for Fig. 2B.