FIG. 8.

Degradation of HO-induced DSB ends. Wild-type (WT) (KSC1623) and mec1Δ (KSC1535) cells carrying pGAL-HO were grown in sucrose and treated with nocodazole. After arrest at G₂/M, the culture was incubated with galactose to induce HO expression. At the indicated time points, aliquots were harvested for DNA preparation. Purified DNAs were fixed to a membrane and probed with ³²P-labeled oligonucleotides each complementary to a 5′-to-3′-degrading strand (A) or a 3′-to-5′-degrading strand (B).