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. Author manuscript; available in PMC: 2014 Oct 14.
Published in final edited form as: Dev Cell. 2013 Sep 26;27(1):10.1016/j.devcel.2013.08.021. doi: 10.1016/j.devcel.2013.08.021

Figure 1.

Figure 1

MCC-specific localization of xCCDC78. (A) Co-localization of C-terminally tagged xCCDC78-GFP with the centriole marker Centrin4-RFP (inlay shows a separate acentriolar foci of xCCDC78-GFP localization not co-localizing with Centrin4) (scale bar, 2μm). (B–C) Localization of N-terminal tagged GFP-xCCDC78 to an acentriolar structure below the apically fused centrioles (i.e. basal bodies) marked with either Centrin4-RFP (B) or γ-tubulin (C) (scale bars, 2μm). (D) TEM micrograph from MTECs depicting a deuterosome (arrowhead) with nascent centrioles forming around it (scale bar, 0.5μm). (E) Localization of GFP-xCCDCD78 to the center of nascent centriole clusters (arrowheads) marked by Centrin4-RFP during the process of centriole biogenesis (scale bar, 2μm). (F) Localization of GFP-hsCCDC78 to centrioles marked with pericentrin and to an acentriolar structure in MTECs (scale bar, 2μm). (G–H) MCC specific localization of GFP-xCCDC78 which is punctate in wild type ciliated epithelia (G) and becomes ubiquitous in MCIN induced ciliated epithelia (H)(scale bars, 10μm)(See also Figure S1 and S2).