Skip to main content
. Author manuscript; available in PMC: 2013 Nov 4.
Published in final edited form as: J Neurosci Res. 2009 Feb 15;87(3):10.1002/jnr.21889. doi: 10.1002/jnr.21889

Fig. 6.

Fig. 6

Inhibition of COX-2, but not MAGL, improves the effect of 2-AG. A: Inhibition of MAGL with NAM. The superfusion of 20 μM NAM (t = 0, bar) did not significantly affect excitatory transmission, and further addition of 30 μM 2-AG (t = 25, bar) elicited an effect comparable to that of 2-AG alone. B: Inhibition of COX-2 with NS398. Top: The selective COX-2 inhibitor NS398 (10 μM) decreased the fEPSPs by 15%, and the subsequent application of 30 μM 2-AG in the continued presence of NS398 decreased fEPSPs by an additional 13%. Bottom: NS398 alone elicited a marked decrease of excitatory transmission and also facilitated the effect of exogenous 2-AG. C: Representative recordings depicting the effect of NS398 and 2-AG in slices pretreated with AM251. With CB1 blocked, NS398 elicited a limited decrease of the fEPSP (about 10%), and subsequent addition of 2-AG did not further alter the synaptic response.