Table I.
16 TCRβ sequences were cloned into a retroviral expression vector and paired to the SMARTA TCRα by fusion PCR. The CDR3β amino acid sequence, length, Vβ usage and Jβ usage for each clone are displayed. Retroviral vectors expressing cloned TCRs were transfected into 293T cells, followed by tetramer staining and calculation of apparent Kd and tetramer off-rates. See also Table S1.
| Clone ID | CDR3β | Length | Vβ | Jβ | Kd (nM) | off-rate (min) |
|---|---|---|---|---|---|---|
| clone 1 | ALQGDNNQAPL | 11 | TRBV31*01 | TRBJ1-5*01 | 5.52 | 824.4 |
| clone 2 | AWRPGTANSDYT | 12 | TRBV31*01 | TRBJ1-2*01 | 88.34 | 709.4 |
| clone 5 | AWSRDSSDYT | 10 | TRBV31*01 | TRBJ1-2*01 | 7.43 | 803.3 |
| clone 7 | AWSLPNYAEQF | 11 | TRBV31*01 | TRBJ2-1*01 | 7.93 | 591.9 |
| clone 10 | ASSDFGQGAERLF | 13 | TRBV13-3*01 | TRBJ1-4*02 | 2.77 | 54.2 |
| clone 13 | ASSDQNNQAPL | 11 | TRBV13-3*01 | TRBJ1-5*01 | 15.58 | 620.5 |
| clone 18 | AWSLWEYAEQF | 11 | TRBV31*01 | TRBJ2-1*01 | 92.37 | 212.0 |
| clone 19 | AWSPGLGVNYAEQF | 14 | TRBV31*01 | TRBJ2-1*01 | 116.20 | 501.6 |
| clone 20 | AWSLIEVF | 8 | TRBV31*01 | TRBJ1-1*01 | 2.33 | 466.5 |
| clone 22 | ASSDHNQANTEVF | 13 | TRBV13-3*01 | TRBJ1-1*01 | 10.86 | 379.0 |
| clone 24 | ASSEMGTGIETLY | 13 | TRBV13-3*01 | TRBJ2-3*01 | 3.31 | 124.4 |
| clone 25 | ASSLAGTGGYEQY | 13 | TRBV29*01 | TRBJ2-7*01 | 6.32 | 151.3 |
| clone 26 | ASSSPGTANYAEQF | 14 | TRBV29*01 | TRBJ2-1*01 | 4.50 | 38.2 |
| clone 27 | ASSPSGTGGYEQY | 13 | TRBV29*01 | TRBJ2-7*01 | 3.57 | 43.3 |
| clone 28 | ASSLHNSGNTLY | 12 | TRBV29*01 | TRBJ1-3*01 | 8.71 | 288.8 |
| clone 29 | AWSLPNSYEQY | 11 | TRBV31*01 | TRBJ2-7*01 | 5.46 | 265.7 |