Table 1.
Single-Nucleotide Polymorphism Primer Sequences
| SNP | S | Primers | Annealing temperature (°C) | Restriction enzyme |
|---|---|---|---|---|
| IκBL −62 | F | F 5′ CACAGTTCACTTCCGTCCTCCAGC 3′a | 58 | PvuII |
| rs2071592 | R 5′ CCTGTGTTTAAGAAGCTCGG 3′ | |||
| IκBL −262 | F | F 5′ CCTCTCTCTGCCAAGTTAGAGGAGGCGCG 3′a | 58 | AciI |
| rs3219184 | R 5′ GGGCCGTCTGAAACCAGAAGACTGG 3′ | |||
| TNFa −238 | F | F 5′ CACTCCCCATCCTCCCTGGTC 3′ | 61 | AvaII |
| rs361525 | R 5′ GGTCCTACACACAAATCAGT 3′ | |||
| TNFa −308 | F | F 5′ AGGCAATAGGTTTTGAGGGCCAT 3′ | 58 | NcoI |
| rs1000629 | R 5′ TCCTCCCTGCTCCGATCCCG 3′ | |||
| LTa +80 | R | F 5′ GAGAGACAGGAAGGGAACAGAG 3′ | 60 | BseYI |
| rs2239704 | R 5′ GTGCTTCGTGCTTTGGACTACCGCTC 3′a | |||
| LTa +252 | R | F 5′ GAGAGACAGGAAGGGAACAGAG 3′ | 60 | NcoI |
| rs909253 | R 5′ GTGCTTCGTGCTTTGGACTACCGCTC 3′a | |||
| FAS −670 | R | F 5′ CTACCTAAGAGCTATCTACCGTTC 3′ | 54 | MvaI |
| rs1800682 | R 5′ GGCTGTCCATGTTGTGGCTGC 3′ | |||
| FASL −844 | F | F 5′ CAGCTACTCAGGAGGCCAAG 3′ | 62 | BsrDI |
| rs763110 | R 5′ GCTCTGAGGGGAGAGACCAT 3′ |
a
Modified primer sequence to create restriction site.
S, sense; F, forward; R, reverse; TNFa, tumor necrosis factors alpha; LTa, lymphotoxin-alpha; SNP, single-nucleotide polymorphism.