TABLE 1.
Kinetic parameters for GFP-Swi6 binding in vivo
| Parameterc | Value ford:
|
||||
|---|---|---|---|---|---|
| clr4-S5, Swi6, eu | WT, Swi6, eu | WT, Swi6, hetero | WT, ΔCD, eu | WT, ΔCSD, eu | |
| kon1 (s−1) | 24.2 | 24.2 | 24.2 | 24.2 | 0.00 |
| koff1 (s−1) | 5.21 | 5.21 | 5.21 | 5.02 | 0.00 |
| kon2 (s−1) | 49.5 | 49.5 | 49.5 | 0.00 | 49.5 |
| koff2 (s−1) | 2.59 | 2.59 | 2.59 | 0.00 | 10.3 |
| kon3 (s−1) | 0.00105 | 0.00105 | 0.00105 | 0.00 | 0.00 |
| koff3 (s−1) | 0.0091 | 0.0091 | 0.0091 | 0.00 | 0.00 |
| kdiff (s−1) | 60 | 60 | 60 | 60 | 60 |
| Pa | 0.06 | 0.18 | 0.99 | 0.06 | 0.99 |
| Swi6 fraction | 0.18 | 0.14 | 0.05 | 0.24 | 0.21 |
| Swi6-H3 fraction | 0.63 | 0.43 | 0.01 | 0.76 | 0.00 |
| Swi6-H3metfast fraction | 0.17 | 0.39 | 0.84 | 0.00 | 0.79 |
| Swi6-H3metslow fraction | 0.02 | 0.04 | 0.10 | 0.00 | 0.00 |
| Residence time for Swi6-H3 (ms) | 192 | 192 | 192 | 199 | NAb |
| Residence time for Swi6-H3metfast (ms) | 385 | 385 | 385 | NA | 97 |
| Residence time for Swi6-H3metslow (s) | 110 | 110 | 110 | NA | NA |
a
P, relative proportion of methylated binding sites.
b
NA, not applicable.
c
Swi6-H3metfast and Swi6-H3metslow, Swi6 bound to methylated H3, fast and slow phases, respectively.
d
Background, version of Swi6 (Swi6, WT; ΔCD, Swi6ΔCD; ΔCSD, Swi6ΔCSD), type of chromatin (eu, euchromatin; hetero, heterochromatin).